2024 Acetaminophen+toxicity+in+rat+and+mouse+hepatocytes+in+vitro - blag0y.ru
WEBResults: APAP from concentrations of 2.5 and 0.75 mmol/L induced a decrease in viability of rat (p < 0.001) and mouse (p < 0.001) hepatocytes (WST-1), respectively. In contrast to rat hepatocytes, there was no activation of caspase-3 in …WEBDec 14, 2016 · APAP from concentrations of 2.5 and 0.75 mmol/L induced a decrease in viability of rat (p < 0.001) and mouse (p < 0.001) hepatocytes (WST-1), respectively.WEBDec 14, 2016 · Results: APAP from concentrations of 2.5 and 0.75 mmol/L induced a decrease in viability of rat (p < 0.001) and mouse (p < 0.001) hepatocytes (WST-1), respectively. In contrast to rat...WEBNov 1, 2012 · Determination of acetaminophen-protein adducts in mouse liver and serum and human serum after hepatotoxic doses of acetaminophen using high-performance liquid chromatography with electrochemical detection.WEBSep 15, 2014 · The most common models used to study APAP hepatotoxicity are mice, rats, primary mouse and human hepatocytes (PMH and PHH, respectively), and hepatoma cell lines. However, the mechanisms of injury and mode of cell death differ.WEBOct 1, 2017 · Objectives: The aim of our work was to compare hepatotoxicity of APAP in rat and mouse hepatocytes in primary cultures. Materials and methods: Hepatocytes isolated from male Wistar rats and C57Bl/6J mice were exposed to APAP for up to 24 h.WEBMar 27, 2020 · Our data support that released HMGB1 from acetaminophen-stressed hepatocytes induced necrosis of neighboring hepatocytes by TLR4-TRIF-RIPK3- pathway.WEBThe data illustrate the pleotropic properties of TNF-α in acetaminophen toxicity and also suggest a redundancy of pathways for hepatocyte regeneration and recovery following acetaminophen toxicity in the mouse.WEBDec 3, 2015 · Organotypic liver culture models for hepatotoxicity studies that mimic in vivo hepatic functionality could help facilitate improved strategies for early safety risk assessment during drug...WEBApr 2, 2015 · The metabolism of acetaminophen (APAP) by different isoforms of cytochrome P450 (mainly CYP2E1) leads to formation of the toxic metabolite NAPQI in hepatocytes. Low levels of NAPQI can be fully converted into the inactive reduced form by the binding of glutathione.WEBApr 8, 2024 · Excessive acetaminophen (APAP) can induce neutrophil activation and hepatocyte death. Along with hepatocyte dysfunction and death, NETosis (a form of neutrophil-associated inflammation) plays a...WEBDec 1, 2009 · The mechanisms participating in its toxic effect are glutathione depletion, oxidative stress and mitochondrial dysfunction. S-adenosylmethionine (SAMe) is the principal biological methyl...WEBWhile the toxic responses can be called forth easily in mice, the human relevancy of these results is questionable. In this study human, rat, and mouse primary hepatocytes were treated with increasing concentrations of APAP, and cell viability was measured by MTT cytotoxicity assay.WEBUptake and conjugation of acetaminophen were studied in isolated rat hepatocytes. Acetaminophen was taken up by the cells and the conjugates formed in the cells were rapidly released from the cells at a constant rate.WEBMar 21, 2018 · Another trigger for the induction of the MPTP in the context of APAP-induced liver injury is lysosomal iron, whose translocation to the mitochondria has been shown to occur in mouse hepatocytes treated with APAP, where it …WEBJun 1, 2008 · In this study, we compared the response of primary human hepatocytes from three donors to primary rat and mouse hepatocytes to the test compound APAP by measuring cell viability using MTT (3- (4,5-dimethylthiazol 2-yl)-2,5 diphenyltetrazolium bromide) reduction assay.WEBJun 1, 2008 · In this study human, rat, and mouse primary hepatocytes were treated with increasing concentrations of APAP, and cell viability was measured by MTT cytotoxicity assay. Pronounced interspecies differences were obtained in cell viability following 24 h of APAP treatment starting at 24 h after seeding (EC 50 : 3.8 mM, 7.6 mM, and 28.2 ...WEBSummary. The present study describes the estimation of acetaminophen. (AAP) toxicity in cultured rat hepatocytes. We used different. concentrations of AAP – 1, 2.5, 5, 10 and 20 mM, to test. influence of AAP on cellular viability, functional capacity and. oxidative status at given time intervals. WST-1 test showed.WEBNov 1, 2012 · Acetaminophen overdose causes severe liver injury only in mice but not in rats. APAP causes hepatic GSH depletion and protein adduct formation in rats and mice. Less protein adducts were measured in rat liver mitochondria compared to mouse. No oxidant stress, peroxynitrite formation or JNK activation was present in rats. The limited ...WEBSep 1, 2014 · The acute toxicity in mice of the four most potent compounds of the series was also assessed. Compound 1b, which has an anaesthetic activity comparable to that of lidocaine, was also...WEBFeb 2, 2017 · Studies in the mouse model, which closely reproduces the human condition, have shown that hepatotoxicity is initiated by formation of a reactive metabolite N -acetyl- p -benzoquinone imine (NAPQI), which depletes cellular glutathione and forms protein adducts on mitochondrial proteins.WEBJun 18, 2024 · Balloon flower root-derived exosome-like nanoparticles (BDEs) have recently been proposed as physiologically active molecules with no cytotoxicity. However, the therapeutic effects of drug-induced hepatotoxicity of BDEs have not been elucidated. BDEs contain a large amount of platycodin D, which is widely known to be effective in …WEBOct 4, 2016 · Acetaminophen (APAP) hepatotoxicity is characterized by an extensive oxidative stress. However, its source, pathophysiological role and possible therapeutic potential if targeted, have been controversially described.WEBJun 1, 2024 · This work presents an advanced model of the liver, which reflects most of the morphologically and metabolically important features of the liver in vivo, namely: three‐dimensionality, cellular composition, presence of extracellular matrix, distribution of individual cell types in the structure of the liver model, high urea and albumin synthesis …
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